- Liquid chromatography- Mass spectrometry (LC-MS)
- HPLC with electrochemical detection (LCEC, amperometry, coulometry)
- HPLC with fluorescence detection
- HPLC with UV/VIS (DAD) detection
- HPLC with conductivity detection
- Immunoassays - EIA, ELISA with UV/VIS, ECL, FL, TRF and LM detection
- Enzyme assays
The experimental armamentarium of Pronexus instrumentation covers the techniques for high-sensitive analysis of endogenous molecules including classic neurotransmitters, their precursors and metabolites, neuromodulators, cytokines, trophic factors, enzyme activities and other neurochemically active molecules.
Pronexus specializes on measuring molecules in small sample volumes, typically 5 – 20 µl.
UHPLC-MS/MS systems for small molecules
Techniques applying ultra high performance liquid chromatography with tandem mass spectrometry allow detection of endogenous molecules and their metabolites, as well as, test compounds and drugs used as positive controls in biological samples including microdialysates, cell lysates, CSF, plasma and brain tissue extracts.
HPLC with electrochemical or fluorescence detection
Techniques optimized for analysis of neurotransmitters, neuromodulators and related molecules in the microdialysates: Serotonin, norepinephrine, dopamine and their metabolites, acetylcholine, GABA, aspartate and glutamate, glycine, physiological amino acids, histamine, adenosine and other purines, cAMP, cGMP, kynurenic acid and other metabolites of the kynurenine pathway, radical oxygen species (ROS), NO2-/NO3- anions.
ELISA and enzymatic spectrophotometric assays
Neuropeptides, hormones, A-beta and tau peptides, synuclein, cytokines, BDNF, NGF, interleukins, enzymes and other clinical biomarkers. Enzymatic assays for small molecules, lactate, pyruvate, glycerol, glucose, urea (CMA Microdialysis Analyser) for clinical microdialysates, spectrophotometric assays for Mg2+, Ca2+ and other ions.
Ultra-sensitive detection of monoamines
Unique fluorescence derivatization/HPLC methods developed for simultaneous determination of noradrenaline, dopamine and serotonin down to 10-11 M [100 atmol/20 µl] and for histamine [300 atmol/20 µl].
We develop and validate methods for measuring drug levels in µl volumes of typical biological matrices including blood, CSF, ISF (microdialysates), urine, micro-dissected tissue extracts, cell lysates and superfusates.